Pass aging
(also known as subculture or splitting cells) involves transferring a
small number of cells into a new vessel. Cells can be cultured for a
longer time if they are split regularly, as it avoids the senescence
associated with prolonged high cell density. Suspension cultures are
easily passaged with a small amount of culture containing a few cells
diluted in a larger volume of fresh media. For adherent cultures, cells
first need to be detached; this is commonly done with a mixture of
trypsin-EDTA; however, other enzyme mixes are now available for this
purpose. A small number of detached cells can then be used to seed a new
culture.
Pass aging
(also known as subculture or splitting cells) involves transferring a
small number of cells into a new vessel. Cells can be cultured for a
longer time if they are split regularly, as it avoids the senescence
associated with prolonged high cell density. Suspension cultures are
easily passaged with a small amount of culture containing a few cells
diluted in a larger volume of fresh media. For adherent cultures, cells
first need to be detached; this is commonly done with a mixture of
trypsin-EDTA; however, other enzyme mixes are now available for this
purpose. A small number of detached cells can then be used to seed a new
culture.
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