Genetic Engineering Info

  Isolation of cells Maintaining cells in culture Cell line cross-contamination Other technical issues Manipulation of cultured c...

      C ells are grown and maintained at an appropriate temperature and gas mixture (typically, 37 °C, 5% CO2 for mammalian cells) i...

Plating density (number of cells per volume of culture medium) plays a critical role for some cell types. For example, a lower plating...

In the case of adherent cultures, the media can be removed directly by aspiration, and then is replaced. Media changes in non-adherent...

Pass aging (also known as subculture or splitting cells) involves transferring a small number of cells into a new vessel. Cells can be c...

Another common method for manipulating cells involves the introduction of foreign DNA by transfection. This is often performed to cause...

It is possible to fuse normal cells with an immortalised cell line. This method is used to produce monoclonal antibodies. In brief, lymp...