Showing posts with label
subculture or splitting cells.
Show all posts
Showing posts with label
subculture or splitting cells.
Show all posts
Pass aging
(also known as subculture or splitting cells) involves transferring a
small number of cells into a new vessel. Cells can be cultured for a
longer time if they are split regularly, as it avoids the senescence
associated with prolonged high cell density. Suspension cultures are
easily passaged with a small amount of culture containing a few cells
diluted in a larger volume of fresh media. For adherent cultures, cells
first need to be detached; this is commonly done with a mixture of
trypsin-EDTA; however, other enzyme mixes are now available for this
purpose. A small number of detached cells can then be used to seed a new
culture.
Pass aging
(also known as subculture or splitting cells) involves transferring a
small number of cells into a new vessel. Cells can be cultured for a
longer time if they are split regularly, as it avoids the senescence
associated with prolonged high cell density. Suspension cultures are
easily passaged with a small amount of culture containing a few cells
diluted in a larger volume of fresh media. For adherent cultures, cells
first need to be detached; this is commonly done with a mixture of
trypsin-EDTA; however, other enzyme mixes are now available for this
purpose. A small number of detached cells can then be used to seed a new
culture.
